Can a bot detection tool detect proxy rotation and location masking on my website?

Yes, proxy rotation and location masking can cause network, location, language, and timing signals to disagree with one another. BotRefund uses the DNS Challenge Blocked check to catch those mismatches and cross-checks them with other browser, device, and behavior data. That makes it harder for a bot to hide behind a rotated proxy or a spoofed location. A single mismatch alone is not enough to prove a bot, though.